By Sara El-Metwally
ISBN-10: 149390714X
ISBN-13: 9781493907144
ISBN-10: 1493907158
ISBN-13: 9781493907151
The advent of subsequent new release Sequencing (NGS) applied sciences led to an enormous transformation within the approach scientists extract genetic info from organic platforms, revealing unlimited perception in regards to the genome, transcriptome and epigenome of any species. besides the fact that, with NGS, got here its personal demanding situations that require non-stop improvement within the sequencing applied sciences and bioinformatics research of the consequent uncooked information and meeting of the whole size genome and transcriptome. Such advancements result in impressive advancements of the functionality and insurance of sequencing and superior caliber for the assembled sequences, however, demanding situations equivalent to sequencing blunders, dear processing and reminiscence utilization for meeting and sequencer particular mistakes continues to be significant demanding situations within the field.
This ebook goals to supply short overviews the NGS box with detailed specialise in the demanding situations dealing with the NGS box, together with info on diversified experimental systems, meeting algorithms and software program instruments, meeting errors correction methods and the correlated challenges.
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References 35 Semiconductor Sequencing Semiconductor sequencing technology, also known as Ion Torrent semiconductor sequencing technology, is a member of the sequencing by synthesis methodologies as the sequencing takes place during the construction of the complementary strand of the single-stranded DNA molecule in question. It involves standard sequencing chemistry but implements a novel nucleotide detection approach. Instead of detecting nucleotides through radioactive or florescent labels, Ion Torrent semiconductor sequencing detects the hydrogen ions released through the DNA polymerization process [27].
2005) Accurate multiplex polony sequencing of an evolved bacterial genome. Science 309 (5741):1728-1732. doi:1117389 20. Margulies M, Egholm M, Altman WE, Attiya S, Bader JS et al. (2005) Genome sequencing in microfabricated high-density picolitre reactors. Nature 437 (7057):376-380. 1038/ nature03959 21. Bentley DR, Balasubramanian S, Swerdlow HP, Smith GP, Milton J et al. (2008) Accurate whole human genome sequencing using reversible terminator chemistry. Nature 456 (7218):53-59. 1038/nature07517 22.
The first genome to be fully sequenced was the bacteriophage φX174 in 1977 by Sanger, while the first “long” genome to be fully sequenced was Epstein–Barr virus (170 Mbp) in 1984 [4]. 2 DNA Sequencing Methods DNA sequencing methods were non-automated techniques until the announcement of the first fully automated DNA sequencing platform by Applied Biosystems in 1987. This heralded a major boom in the field which led to the sequencing of several S. 1007/978-1-4939-0715-1_3, © The Authors 2014 29 30 3 First- and Next-Generations Sequencing Methods model organisms and the great human genome-sequencing project [5].
Next Generation Sequencing Technologies and Challenges in Sequence Assembly by Sara El-Metwally
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