Download e-book for iPad: Medical Biotechnology by Bernard R. Glick

By Bernard R. Glick

ISBN-10: 155581705X

ISBN-13: 9781555817053

The destiny is now―this groundbreaking textbook illustrates how biotechnology has greatly replaced the way in which we predict approximately well-being care

Biotechnology is offering not just new items to diagnose, hinder, and deal with human sickness yet solely new ways to quite a lot of tricky biomedical demanding situations. as a result of advances in biotechnology, enormous quantities of recent healing brokers, diagnostic exams, and vaccines were built and come available to buy.

In this jargon-free, easy-to-read textbook, the authors demystify the self-discipline of clinical biotechnology and current a roadmap that offers a basic figuring out of the wide-ranging ways pursued via scientists to diagnose, hinder, and deal with health conditions.

Medical Biotechnology is written to coach premed and scientific scholars, dental scholars, pharmacists, optometrists, nurses, nutritionists, genetic counselors, clinic directors, and people who are stakeholders within the realizing and development of biotechnology and its impression at the perform of contemporary drugs.

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Sample text

5) Any unbound secondary antibody is washed away, and a colorimetric (or chemiluminescent) reaction is carried out. The reaction can occur only if the secondary antibody, which is attached to an enzyme (E) that performs the reaction, is present. (6) A colony on the master plate that corresponds to a positive response on the matrix is identified. Cells from the positive colony on the master plate are subcultured because they may carry the plasmid–insert DNA construct that encodes the protein that binds the primary antibody.

The poly(A) tail provides the means for separating the mRNA fraction of a tissue from the more abundant ribosomal RNA (rRNA) and transfer RNA (tRNA). Short chains of 15 thymidine residues (oligodeoxythymidylic acid [oligo(dT)]) are attached to cellulose beads, and the oligo(dT)–cellulose beads are packed into a column. Total RNA extracted from eukaryotic cells or tissues is passed through the oligo(dT)– cellulose column, and the poly(A) tails of the mRNA molecules bind by base-pairing to the oligo(dT) chains.

All steps in a PCR cycle are carried out in an automated block heater that is programmed to change temperatures after a specified period of time. 1. Denaturation. The first step in a PCR is the thermal denaturation of the double-stranded DNA template to separate the strands. This is achieved by raising the temperature of a reaction mixture to 95°C. , Taq DNA polymerase, isolated from the bacterium Thermus aquaticus), and four deoxyribonucleotides. 2. Annealing. For the second step, the temperature of the mixture is slowly cooled.

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Medical Biotechnology by Bernard R. Glick


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