By Dr. Stephen F. Goodwin
ISBN-10: 0123748364
ISBN-13: 9780123748362
Genes engage with surroundings, event, and the biology of the mind to form an animal's habit. This most recent quantity in Advances in Genetics, geared up in keeping with the main known version organisms, describes the most recent genetic discoveries relating to neural circuit improvement and job. * Explores most up-to-date issues in neural circuits and behaviour examine in zebrafish, drosophila, c.elegans, and mouse types* comprises equipment for trying out with moral, felony, and social implications* severely analyzes clients destiny clients
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Additional resources for Genetic Dissection of Neural Circuits and Behavior
Sample text
Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature 450, 56–62. , Beverloo, H. , Allak, B. , and Jonkers, J. (2001). Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells. Proc. Natl. Acad. Sci. USA 98, 9209–9214. , Jones, M. , Bliss, T. , Vanhoose, A. , Kandel, E. , Winder, D. , and Mansuy, I. M. (2001). Inducible and reversible enhancement of learning, memory, and long-term potentiation by genetic inhibition of calcineurin.
Physiol. 334, 33–46. Conklin, B. , Hsiao, E. , Forsayeth, J. , Guettier, J. , Chang, W. , McCarthy, K. , Nissenson, R. , et al. (2008). Engineering GPCR signaling pathways with RASSLs. Nat. Methods 5, 673–678. Coons, A. , Creech, H. , and Jones, R. N. (1941). Immunological properties of an antibody containing a fluorescent group. Proc. Soc. Exp. Biol. Med. 47, 200–202. Crabbe, J. , and Dudek, B. C. (1999). Genetics of mouse behavior: Interactions with laboratory environment. Science 284, 1670–1672.
Immunohistochemistry was first described in 1941 by Coons et al. (1941) (for review see LeDoux, 2000; Ramos-Vara, 2005) and relies on the binding of specific antibodies to antigens of interest. The antigen– antibody binding is visualized by histochemical reactions or fluorochromes. The development of phospho-specific antibodies that bind specifically to the activated form of a protein increased the potency of this technique. One of the most frequently used phospho-sites to map neuronal activity is the serine 133 residue of the CREB, a transcription factor that is crucial for memory consolidation.
Genetic Dissection of Neural Circuits and Behavior by Dr. Stephen F. Goodwin
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