By Zbigniew Darzynkiewicz, John Paul Robinson, Harry A. Crissman, Leslie Wilson, Paul T. Matsudaira
ISBN-10: 0122030516
ISBN-13: 9780122030512
ISBN-10: 0125641427
ISBN-13: 9780125641425
Flow Cytometry, moment variation offers a whole and accomplished quantity laboratory consultant and reference for using the most up-tp-date equipment in movement cytometry pattern guidance and research. those crucial thoughts are defined in a step by step structure, supplemented via explanatory sections and trouble-shooting assistance. The tools are obtainable to all researchers and scholars in biomedical technology and biology who needs to use movement cytometry to split and study cells. Key positive factors * thoroughly revised and tremendously elevated because the ebook of the 1st version in 1990 * equipment disguise telephone dying and phone cycle analyses useful, handbook-style presentation works in lab or school room * particular finished methodological insurance * colour plates illustrate thoughts * In-depth therapy of approaches, together with an outline of every technique: * Theoretical foundations * severe elements * attainable pitfalls * Written by way of authors with broad adventure who: * built or converted the recommendations * Describe their event with assorted tools and functions to various mobile platforms * Are the who is Who in movement Cytometry
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Additional info for Flow Cytometry Part A
Example text
Assays of Cell Viability B. Reagents 1. 1 M citric acid. 8. 2 . Hanks’ buffered salt solution (HBSS). 3 . Fixative: 70% ethanol. C . Procedure 1. , add 1 ml of cells suspended in HBSS containing approximately lo6 cells into 9 ml of 70% ethanol in tube) on ice. Cells in fixative (ethanol) can be stored at -20°C for up to 1 week. 2. Centrifuge cells, decant ethanol, suspend cells in 10 ml of HBSS, and centrifuge. 3. 0 ml of the phosphate-citric acid buffer (reagent No. 1). 2 ml or none) buffer if DNA degradation in Ap cells is extensive and DNA extraction effective, so the Ap cells are well separated from G , cells.
Koss, L. , Sherman, A. , and Elequin. F. (1984). A m . J. Clin. Path. 82, 559-564. Hefley, T. , Stern, P. , and Brand, J . S. (1983). Exp. Cell Res. 149, 227-236. Kallioniemi, 0. P. (1988). Cytornetry 9, 164-169. McDivitt, R. , Stone, K. , and Meyer, J. S. (1984). Cancer Res. 44, 2628-2633. Pallavicini, M. , Folstad, L. J . , and Dunbar, C. (1981). Cytometry 2, 54-58. Remvikos, Y . , and Zajdela, A. (1988). Cancer (Philadelphia)61, 1629-1634. Rong, G. , Grimm, E. A , , and Sindelar, W. F. (1985).
Cytornetry 9, 164-169. McDivitt, R. , Stone, K. , and Meyer, J. S. (1984). Cancer Res. 44, 2628-2633. Pallavicini, M. , Folstad, L. J . , and Dunbar, C. (1981). Cytometry 2, 54-58. Remvikos, Y . , and Zajdela, A. (1988). Cancer (Philadelphia)61, 1629-1634. Rong, G. , Grimm, E. A , , and Sindelar, W. F. (1985). J. Surg. Oncol. 28, 131-133. Slocurn, H. , Pavelic, 2. , Rustum, Y. , Kanter, P. , and Nowak, N . J. (1981). Cancer Res. 41, 1428-1434. Vindel$ov, L. , Christensen, I. , and Nissen, N. I.
Flow Cytometry Part A by Zbigniew Darzynkiewicz, John Paul Robinson, Harry A. Crissman, Leslie Wilson, Paul T. Matsudaira
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