By A. Neuberger and L.L.M. Van Deenen (Eds.)
ISBN-10: 0444803033
ISBN-13: 9780444803030
ISBN-10: 0444806490
ISBN-13: 9780444806499
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Example text
The origins of the rnultiplet structure in the ‘Hspectrum of an ethyl group, and rules of binomial distribution (from [61]). 19 TABLE 3 Typical ranges of spin-spin coupling constants -12 to -15 2-14 (7 if free rotation) 10 (cis) 17 (trans) 1-10 0-3 11cL130 -5 to +s 7z2 61 85-95 (peptides) 15-23 (peptides) 15-25 4cL50 5-20 -280 to -350 It has been possible to correlate molecular structure and stereochemistry, including parameters such as electron distributions and bond angles, to observed coupling constants.
All these sequences include a (90") preparation pulse which flips the magnetization of the spin system into the xy plane. The evolution period is then the period of free precession of the spin system in the xy plane, in the Hahn spin echo experiment (Fig. 6).
The fact that in vivo spectral linewidths are broad (tens of Hz), relative to the change in chemical shift per pH unit, also contributes to inaccuracies in pH measurement. It is clear from this discussion that accurate pH measurements using NMR can only be obtained if careful controls are made. Figure 18. 1 M KCI (m). Reprinted, with permission, from [26], 0 1981, American Chemical Society. 33 (e) Compartmentation of metabolites It has long been recognised that metabolites, and metabolic activities, are distributed non-uniformly within cells.
Modern Physical Methods in Biochemistry by A. Neuberger and L.L.M. Van Deenen (Eds.)
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