By Alister G. Craig and Jörg D. Hoheisel (Eds.)
ISBN-10: 0125215274
ISBN-13: 9780125215275
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Additional info for Genomic and Functional Analyses
Example text
We have found that the illumination conditions required to acquire high contrast images of plaques are entirely different to the set-up needed for colonies. I. Colony illumination Back lighting alone is not helpful if colour or density discrimination is requird colonies simply show up as dark circular masses. The simplest way to achieve dark field illumination is to put a black mat between the light box and the Petri dish, such that the lighting comes from the sides (Figure 3). Looking from above, there is no direct light in the field of view, giving a black background.
In the case of FlexysTMthere is sufficient drop below the bed of the robot to house the light box enclosure. In future systems we might not have this luxury. We investigated two alternatives: fibre optic mats and eledroluminescent panels. (a) Fibre optic mats Fibre optic guides deliver "cold light from an incandescent or other source, and are useful when illuminating heat sensitive or inaccessible locations. A web of fibre optic material can be netted into a flat mat. The fibres can be arranged to leak light out of the planar surface, offering the opportunity to construct a flat light emitting surface.
The illumination is sufficiently close to parallel to give a significant contrast improvement over a diffuse transilluminator when imaging plaques. The lighting generated is completely uniform. When viewed normal to the direction of illumination, the maximum contrast improvement over a uniformly diffusing surface is given by the ratio of the illumination of the spherical cap of the diffusion cone, to that of the entire hemisphere (Figure 10). For a hemisphere: r E, = A lux 2n r For the spherical cap: 29 ,, Brightness enhancement film Diffuse 7 Fluorescent tube light source Diffusing screen Figure 9.
Genomic and Functional Analyses by Alister G. Craig and Jörg D. Hoheisel (Eds.)
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