New PDF release: Gene Cloning and DNA Analysis: An Introduction (Brown,Gene

By T. A. Brown

ISBN-10: 1405111216

ISBN-13: 9781405111218

As an introductory textual content on its topic, the ebook is definitely written. With copious diagrams which are effortless to appreciate and that illustrate key rules. A advantage of the publication is the readability of the textual exposition, strengthened via these diagrams.

The textual content is additionally really brand new during this quickly altering box. With the nice insurance of many themes. together with the seminal Polymerase Chain response, that's the foundation of a lot else. one can find that genomics/biotechnology is now a pragmatic and quantitative technology. With lots last to be understood, to make sure. however the booklet exhibits that we've got strong instruments to test with, to lessen our lack of expertise.

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Additional info for Gene Cloning and DNA Analysis: An Introduction (Brown,Gene Cloning and DNA Analysis)

Sample text

6): (1) The phage particle attaches to the outside of the bacterium and injects its D~A chromosome into the cell. (2) The phage DNA molecule is replicated, usually by specific phage enzymes coded by genes on the phage chromosome. g. g. M13). '(capsid) Head (contains DNA) DNfJ.. 6 The general pattern of infection of a bacterial cell by a bacteriophage. The phage attaches to the bacterium and injects its DNA Phage DNA molecules 2 The phage DNA molecule is replicated Capsid components I , 3 Capsid components are synthesized, new phage particles are assembled and released New phage particles CD ~ (3) Other phage genes direct synthesis of the protein components of the capsid, and new phage particles are assembled and released from the bacterium.

Medium Component M9 medium Na2HP04 KH2P0 4 NaCl NH4 C! 1 is rather different. Luria­ Bertani (LB) is a complex or undefined medium, meaning that the precise identity and quantity of its components are not known. This is because two of the ingredients, tryptone and yeast extract, are complicated mixtures of unknown chemical compounds. Tryptone in fact supplies amino acids and small peptides, while yeast extract (a dried preparation of partially digested yeast cells) provides the nitrogen requirements, along with sugars and inor­ ganic and organic nutrients.

Although the basic steps in DNA purification are the same whatever the organism, some modifications may have to be introduced to take account of the special fea­ tures of the cells being used. Obviously growth of cells in liquid medium is appropriate only for bacte­ ria, other microorganisms, and plant and animal cell cultures. The major mod­ The ifications, however, are likely to be needed at the cell breakage chemicals used for disrupting bacterial cells do not usually work with other organisms: lysozyme, for example, has no effect on plan t cells.

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Gene Cloning and DNA Analysis: An Introduction (Brown,Gene Cloning and DNA Analysis) by T. A. Brown


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