Download e-book for kindle: Topics in Fluorescence Spectroscopy: Probe Design and by Joseph R. Lakowicz (auth.), Joseph R. Lakowicz (eds.)

By Joseph R. Lakowicz (auth.), Joseph R. Lakowicz (eds.)

ISBN-10: 0306447843

ISBN-13: 9780306447846

ISBN-10: 0306470608

ISBN-13: 9780306470608

Time-resolved fluorescence spectroscopy is favourite as a study software in bioch- istry and biophysics. those makes use of of fluorescence have ended in huge wisdom of the constitution and dynamics of organic macromolecules. this knowledge has been won through experiences of phenomena that have an effect on the excited nation, corresponding to the neighborhood atmosphere, quenching approaches, and effort move. themes in Fluorescence Spectroscopy, quantity four: Probe layout and Chemical Sensing displays a brand new pattern, that's using time-resolved fluorescence in analytical and scientific chemistry. those rising purposes of time-resolved fluorescence are the results of persevered advances in laser detector and desktop expertise. for example, pho- multiplier tubes (PMT) have been formerly cumbersome units. Miniature PMTs are actually to be had, and the functionality of easier detectors is consistently bettering. there's additionally substantial attempt to advance fluorophores that may be excited with the red/ne- infrared (NIR) output of laser diodes. utilizing such probes, you can still with ease think small time-resolved fluorometers, even hand held units, getting used fordoctor’s workplace or domestic future health care.

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Extra info for Topics in Fluorescence Spectroscopy: Probe Design and Chemical Sensing

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40(4), 451–456 (1992). B. G. Pinsky, J. J. Ladasky, J. R. Lakowicz, K. Berndt, and R. A. Hoffman, Phase-resolved fluorescence lifetime measurements for flow cytometry, Cytometry 14, 123–135 (1993). J. A. Steinkamp and H. A. Crissman, Resolution of fluorescence signals from cells labeled with fluorochromes having different lifetimes by phase-sensitive flow cytometry, Cytometry 14, 210–216 (1993). J. R. Lakowicz, H. Szmacinski, K. Nowaczyk, K. Berndt, and M. L. Johnson, Fluorescence lifetime imaging, Anal.

Recognition Based on Cation Control of the Proximity between Two Fluorophores, or a Fluorophore and a Quencher The basic principle of this method of recognition is a cation-induced conforma- tional change bringing closer together (or moving away) two moieties able to interact and induce photophysical effects: excimer or exciplex formation (or disappearance), electronic energy transfer and quenching. 1. 2. about compound 8. 12 provides two interesting examples of anthracenocrown ethers. Compound 25(54) exhibits a fluorescence spectrum composed of the characteristic monomer and excimer bands.

14. E. U. Akkaya and J. R. Lakowicz, Styryl-based wavelength ratiometric probes: A new class of fluorescent calcium probes with long wavelength emission and a large stokes’ shift, Anal. Biochem. 213, 285–289 (1993). Bioprobes 13, 3–4 (1991). 15. J. E. Whitaker, R. P. Haugland, and F. G. Prendergast, Spectral and photophysical studies of 16. 17. 18. benzo[c]xanthene dyes: Dual emission pH sensors, Anal. Biochem. 194, 330–344 (1991). J. R. Lakowicz, H. Szmacinski, and M. L. Johnson, Calcium imaging using fluorescence lifetimes and long-wavelength probes, J.

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Topics in Fluorescence Spectroscopy: Probe Design and Chemical Sensing by Joseph R. Lakowicz (auth.), Joseph R. Lakowicz (eds.)


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