By Jerome P Richie; Anthony V D'Amico
ISBN-10: 0721600034
ISBN-13: 9780721600031
ISBN-10: 0721694268
ISBN-13: 9780721694269
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78. 79. 80. 81. 82. 83. 84. 85. biochemically transformed with herpes virus genetic information. Virology 1980; 102:420. Li Y, McCadden J, Ferrer F, et al: Prostate-specific expression of the diphtheria toxin A chain (DT-A): studies of inducibility and specificity of expression of prostatespecific antigen promoter-driven DT-A adenoviralmediated gene transfer. Cancer Res 2002; 62:2576-82. Hoganson DK, Batra RK, Olsen JC, Boucher RC: Comparison of the effects of three different toxin genes and their levels of expression on cell growth and bystander effect in lung adenocarcinoma.
Liposomal vectors in current use are complexes of cationic lipids and plasmid DNA. Although relatively safe and immunologically inert, liposomal vectors require nuclear localization for access to target cell transcription factors. ) Chapter 2 Gene Therapy for Urologic Cancer: Basic Principles, Prospects, and Limitations 25 EMERGING VECTORS AND OTHER GENE DELIVERY SYSTEMS Figure 2-6 Nonviral gene transfer via particle bombardment (gene gun). In the helium pulse gene gun, motive force is generated by release of a high-pressure burst of helium gas from a reservoir (A) at a preset pressure (150-700 psi).
101,102 Molecular conjugate formation has successfully conferred altered and refined specificity to adenoviral, liposomal, and retroviral vectors, alike. 103–106 Restricted Transgene Expression: Transcriptional Targeting An alternative to vector targeting at the target cell binding level is to limit expression of therapeutic genes by regulating transcription with a promoter region having either tissue restricted activity, or preferential activity in malignant cells. 109,110 Cloning of therapeutic cytotoxic genes, such as HSV-tk, into analogous vectors using tyrosinase promoter has been shown to inhibit tumor growth in melanoma animal models111; analogous vectors to target prostate cancer are under development.
Urologic oncology by Jerome P Richie; Anthony V D'Amico
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