By John A. Sykes (auth.), Jørgen Fogh (eds.)
ISBN-10: 1475716478
ISBN-13: 9781475716474
ISBN-10: 1475716494
ISBN-13: 9781475716498
The research of cultured human tumor cells is a most blatant process in experimental human melanoma study. for plenty of concepts in virology, immunology, biochemistry, and biophysics, for instance, quite a lot of cells might be required and such amounts tend to be supplied purely while the cultures become demonstrated telephone traces; while this occurs, thorough characterization additionally turns into attainable. the improvement of mobilephone strains, for this reason, is of major value. contemporary significant advances in examine with animal cellphone platforms see m to be a prologue for current and destiny efforts directed towards paintings with human tumor cells in tradition. Conceivably, the main major leads to melanoma study might strengthen from paintings with such cells, and so the time appeared correct to outline the current country of our wisdom. this can be the 1st booklet committed solely to the topic: human tumor cells in vitro. even supposing so me of the basic elements within the cultivation of human tumor ceIls, and the level to which they signify human melanoma in vivo are nonetheless uncertain, I requested some of the best investigators during this region of study to gather and review past and current contributions, and to provide their ideas at the inquiries to which solutions aren't but to be had. a few of the chapters are taken with concepts of cultivation. Cultures from a few forms of tumors have grown weIl; in lots of instances they've got given upward push to tested telephone lines.
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Johnson, A. , and Moran, M. , 1966, Comparison of several methods for isolation of rat peritoneal mast cells, Proc. Soc. Exp. Biol. Med. 123:886-889. , Kasten, F. , 1970, Isolation of viable neurons from embryonic spinal ganglia by centrifugation through albumin gradients, Exp. Cell Res. 63:189-192. Lasfargues, E. , 1958, Cultivation of human breast carcinomas, J. Natl. 21:1131-1147. , 1971, A centrifugation technique for cytochemical preeparations, J. Histochem. Cytochem. 19:758-760. Mateyko, G.
Development of Media for the Cultivation of Cancer Cells The trend in media for tissue cell cultures has been from the complete body fluid (Harrison, 1907) to the complete synthetic media (White, 1946, 1949). Over 100 different media have been advocated in the last 60 years (Waymouth, 1972) for both general and special needs. Most media in use today for tissue cell cultivation are ablend of body fluids, usually serum, and synthetic ingredients. , 1968). , 1972). Thus tissue culture media design is still essentially empirical.
1955; FIG. 6. Final transition of SW-48 into a complete monolayer of epithelial-like cells. This culture had been passed 15 times in 18 months. 44 ALBERT LEIBOVITZ F~gh and Lund, 1957). P AP medium appears to enchance the growth and longevity of cancer-like cells; an unexpected dividend is that fibroblasts grow slower in this medium than in previous formulas. However, collagenase medium is still necessary when they start to dominate the culture. Our attempts to develop a medium for the growth of newly isolated cancer cells are empirical at this stage.
Human Tumor Cells in Vitro by John A. Sykes (auth.), Jørgen Fogh (eds.)
by Paul
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