Download PDF by M.H.V. Van Regenmortel and S. Muller (Eds.): Synthetic Peptides as Antigents

By M.H.V. Van Regenmortel and S. Muller (Eds.)

ISBN-10: 008085897X

ISBN-13: 9780080858975

ISBN-10: 0444821759

ISBN-13: 9780444821751

This most modern version to the Laboratory concepts sequence offers present state-of-the-art use of artificial peptides in molecular biology and functional protocols on tips on how to conjugate peptides, immunize animals with peptides and visual display unit immune responses to peptides in vitro. It offers historical past info on antigenic specificity, prediction of antigenic websites in proteins and purposes of peptides in immunology and virology, as probes in prognosis and as vaccines. The booklet additionally describes antigenicity of proteins and techniques to localize antigenic websites in addition to equipment for predicting epitoxes, and provides precise protocols for peptide-carrier conjugation, immunization with peptides, and peptide immunoassays. the amount additionally describes commonplace use of antipeptide antibodies in molecular and mobile biology in addition to using peptides within the prognosis of viral infections and autoimmune illnesses, and using peptides as strength man made vaccines. a good variation to an exceptional sequence, to be had in hardbound and paperback.

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Extra info for Synthetic Peptides as Antigents

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4. Binding studies With adpeptide antibodies In this approach, peptides are used for immunizing animals and the resulting antipeptide antibodies are tested for their capacity to crossreact with the intact protein. A positive cross-reaction is taken as evidence that the peptide approximates to an epitope of the protein. , 1983) that immunization with peptides leads to a high frequency of induction of antibodies capable of recognizing the native protein, this is no longer believed to be true. , 1988; Jemmerson, 1987; Kennel, 1982; Soderquist and Walton, 1980; Spangler, 1991).

Each model has some merit and highlights a particular feature of the epitopes. However, each model also inevitably gives a distorted view of the dynamic nature of epitopes (Van Regenmortel, 1996). , 1983) that cross-reaction studies with Mabs are likely to lead to erroneous assignments of discontinuous epitopes, because investigators allegedly tend to disregard the possibility that the substitutions alter the conformation of the protein by long-range effects and may affect epitopes elsewhere in the protein.

1986). The mimotope approach disregards the sequence of the protein under study and analyzes the ability of peptides of increasing size to bind to antiprotein antibodies. Starting with the 400 possible dipeptides obtainable with the 20 common amino acids, the dipeptide possessing the highest binding activity is selected and lengthened on either side with the residues that produce the greatest increment in antigenic reactivity. In this way, hexapeptides can be constructed which possess considerable binding capacity for a particular antiprotein antibody.

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Synthetic Peptides as Antigents by M.H.V. Van Regenmortel and S. Muller (Eds.)


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