By Marie-Cécile Caillaud
ISBN-10: 1493931415
ISBN-13: 9781493931415
ISBN-10: 1493931423
ISBN-13: 9781493931422
This quantity goals to provide a wide panel of strategies for the examine of Plant mobilephone department. Plant mobilephone department: tools and Protocols captures easy experimental protocols which are well-known to review plant cellphone department methods, in addition to extra cutting edge systems. Chapters are break up into 5 components protecting numerous diverse element of plant mobilephone department resembling, mobilephone cultures for mobile department stories, mobilephone cycle development and mitosis, imaging plant mobile department, cellphone department and morphogenesis, and cytokinesis. Written for the Methods in Molecular Biology sequence, chapters comprise introductions to their respective issues, lists of the required fabrics and reagents, step by step, with no trouble reproducible laboratory protocols, and pointers on troubleshooting and fending off recognized pitfalls.
Authoritative and functional, Plant mobile department: tools and Protocols is a priceless software for the research of plant telephone department at either the mobile and molecular degrees, and within the context of plant development.
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Extra resources for Plant Cell Division: Methods and Protocols
Sample text
C) The cells of LBA4404 carrying pCAMBIA1390/ABD2-GFP are themselves fluorescent. (d) Bright-field picture of the Agrobacterium cells seen in (c). (e) Typical result obtained after 2–3 days of incubation. The projection of a confocal z-stack shows a tobacco BY-2 cell with labeled F-actin. Agrobacterium is fluorescent as well (arrowhead) and some of its cells are directly attached to the plant cell. Scale bars: (c, d) 2 μm; (e) 10 μm 22 Henrik Buschmann 8. Incubate at 25 °C in the dark for 2 days.
7. In case of LBA4404 carrying the CaMV35S::ABD2-GFP construct, nearly 100 % of Agrobacterium cells should be fluorescent in the 2-day-old coculture. Acknowledgment I am grateful to Sabine Zachgo for giving me the possibility to carry out research in the Botany Department, Osnabrück University, Germany. Special thanks to Elison Blancaflor, Noble Foundation, USA, for providing the ABD2-GFP construct in pCAMBIA1390. Many thanks to Clive Lloyd, John Innes Centre, UK, for supporting the development of the transformation technique.
9. After 2 days, scrape off a small amount of tobacco cells from the coculture plate (solid Paul’s medium) and mix this with a drop of Paul’s medium on a microscope slide. Apply cover slip and mount slide on confocal microscope. 10. Observe tobacco BY-2 cells (using the 63× oil objective) in bright-field. Observe Agrobacterium, some of which are attached to the BY-2 cell’s surface. 11. Switch to FITC mode (using the mercury lamp). Observe fluorescent bacteria (Fig. 1c, d; see Note 7). Screen for tobacco BY-2 cells with labeled F-actin (Fig.
Plant Cell Division: Methods and Protocols by Marie-Cécile Caillaud
by Edward
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