By Alan S. Gerstein
ISBN-10: 0471223905
ISBN-13: 9780471223900
ISBN-10: 0471379727
ISBN-13: 9780471379720
So much examine within the existence sciences comprises a middle set of molecular-based apparatus and strategies, for which there's no scarcity of step by step protocols. still, there is still a very excessive variety of inquiries positioned to advertisement technical help teams, particularly concerning difficulties.
Molecular Biology challenge Solver: A Laboratory Guide asks the reader to contemplate the most important questions, such as:
- Have you chose the main applicable examine strategy?
- Have you pointed out the problems serious on your profitable program of a technique?
- Are you acquainted with the constraints of a given technique?
- When should still universal procedural ideas of thumb no longer be applied?
- What concepts may you observe to solve a problem?
A specified question-based structure experiences universal assumptions and laboratory practices, with the purpose of delivering a company realizing of the way suggestions and systems paintings, in addition to the best way to steer clear of difficulties. a few significant concerns explored by means of the book's professional members include:
- Working correctly with organic samples and radioactive materials
- DNA and RNA purification
- PCR
- Protein and nucleid acid hybridization
- Prokaryotic and eukaryotic expression systems
- Properly utilizing and retaining laboratory equipment
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Extra resources for Molecular biology problem solver: a laboratory guide
Example text
The Roles of Reporting . . . . . . . . . . . . . . . The Rewards . . . . . . . . . . . . . . . . . . . . . Bibliography . . . . . . . . . . . . . . . . . . . . . 2 2 2 2 3 4 4 4 4 5 5 5 5 5 6 7 7 8 9 9 1 THE PROJECT If You Don’t Define the Project, the Project Will Define You One of the first and toughest questions researchers must answer to foster success in the lab is: What do I have to accomplish? This requires you to understand your purpose to the larger task at hand.
Are Identical Products Manufactured Identically? . . . . Will a Company Inform You When They Change the Product? . . . . . . . . . . . . . . . . . . . . How Can You Work Most Efficiently—and Pleasantly— with a Sales Representative? . . . . . . . . . . . . . . What Can a Sales Rep Do for You? . . . . . . . . . . What Should You Expect from a Sales Rep? . . . . . . How Can You Get What You Want from a Sales Rep? . . Ordering a Custom Product .
One desire is to uncover the truth no matter the outcome (the noblest case), while a second is to achieve our personal goals (the practical case). Research is not done in a vacuum and inherently contains biases. Consider these conscious and subconscious factors when you and your colleagues interpret data and offer conclusions. Ideally there will be only one tenable interpretation of the data, but this is a rare outcome. Providing a fair treatment of the various interpretations in the report should lead to a dispassionate discussion that produces a consensus next step if not a conclusion.
Molecular biology problem solver: a laboratory guide by Alan S. Gerstein
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