William W. Christie's Gas Chromatography and Lipids: A Practical Guide PDF

By William W. Christie

ISBN-10: 095141710X

ISBN-13: 9780951417102

Fuel chromatography was once first constructed via lipid analysts and lipid analysts were on the vanguard within the improvement of the strategy.

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Read e-book online Gas Chromatography and Lipids: A Practical Guide PDF

Gasoline chromatography was once first built through lipid analysts and lipid analysts were on the leading edge within the improvement of the approach.

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W. J. Barnes & Assoc. (Oily Press) A large number of books have been published on the theory and practice of chromatography, and the author has found those cited to be of particular value [267,314,427,444,728,791]. In addition, there have been a number of review articles or books on the application of this theory to specific lipid problems, and these are listed in the appropriate Chapters below. B. THEORY 1. Some basic considerations Most analysts appear to manage perfectly well with only a smattering of knowledge of the theory of gas chromatography.

In many analyses of lipids, the results will be presented simply in terms of the relative proportions (expressed in percentage terms) of each component. There may also be instances, where the absolute amount of each component must be measured. This is best accomplished by adding a precise quantity of an internal standard, ideally a substance which resembles those to be analysed in its chromatographic properties, but does not occur naturally in the sample. If possible, this should be added when the tissue is first extracted, so that it is carried through the extraction, group separation and derivatization steps, as well as through chromatography.

G. QUANTITATIVE ANALYSIS As no sample splitting occurs, there should be no discrimination of sample components. The sample is not vaporised instantly, so it is not stressed as much as in other procedures. In consequence, a high degree of precision can be attained in quantitative analyses In all aspects of chromatographic analysis, from sample preparation to the separation process itself, there are opportunities for errors to occur. Assuming everything that is possible has been done to minimise this, there can be little doubt that electronic digital integration is the most accurate and reproducible, not to mention the most rapid and convenient, means of quantifying chromatographic peaks.

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