By Andrés Illanes, Andrés Illanes
ISBN-10: 1402083602
ISBN-13: 9781402083600
ISBN-10: 1402083610
ISBN-13: 9781402083617
This publication used to be written with the aim of delivering a legitimate foundation for the layout of enzymatic reactions according to kinetic ideas, but additionally to offer an up-to-date imaginative and prescient of the potentials and barriers of biocatalysis, in particular with recognize to fresh purposes in procedures of natural synthesis. the 1st 5 chapters are based within the type of a textbook, going from the elemental rules of enzyme constitution and serve as to reactor layout for homogeneous structures with soluble enzymes and heterogeneous platforms with immobilized enzymes. The final bankruptcy of the booklet is split into six sections that symbolize illustrative case experiences of biocatalytic strategies of commercial relevance or strength, written via specialists within the respective fields.
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Extra info for Enzyme Biocatalysis: Principles and Applications
Sample text
2005), representing one of the most successful cases of industrial application of hydrolytic enzymes in bioprocesses. Urease is industrially used to remove urea from alcoholic beverages in Japan (Kodama 1996). Removal of urea precludes the formation of toxic ethylcarbamate during fermentation, which is particularly relevant in the production of sake. The continuous process with immobilized Lactobacillus fermentum urease has been developed and optimized (Matsumoto 1993). Beyond hydrolytic enzymes there are some other enzymes of significant industrial impact.
This requires significant increase in specific activity and production efficiency. Optimized combinations of the different cellulase fractions have been successfully employed (Baker et al. 1998) and promising results have been obtained in cellulase improvement by genetic and protein engineering techniques (Godbole et al. 1999; Sch¨ulein 2000). These advances go in parallel with those in the field of plant genetic engineering where fast growing species with lower lignin and higher cellulose content, and ligninase self producing species are promising developments (Sticklen 2006).
Ionic liquids are highly polar solvents composed usually by a rather simple anion and a complex organic cation (van Rantwijk et al. 2003). Ionic liquids have negligible vapor pressure which is quite important from an environmental perspective. In addition, they are less harmful than organic solvents and form biphasic systems with them, being attractive for lipase catalyzed reactions (Mori et al. 2005). Increased enzyme enantioselectivity (Liu et al. 2005; Durand et al. 2007) and stability (Machado and Saraiva 2005; Ulbert et al.
Enzyme Biocatalysis: Principles and Applications by Andrés Illanes, Andrés Illanes
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