By Grant Jensen
This quantity is devoted to an outline of the tools, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the tips, intrumentation, and techniques underlying all cryo-EM ways which permit practictioners to simply stream among them. inside every one part, the articles are ordered in accordance with the commonest symmetry of the pattern to which their tools are applied.* Includes time-tested core methods and new innovations applicable to any researcher * equipment incorporated are important to either established researchers and beginners to the sphere * appropriate historical past and reference details given for methods can be utilized as a consultant
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Additional info for Cryo-EM, Part C: Analyses, Interpretation, and Case Studies
Functional Glycomics Edited by MINORU FUKUDA VOLUME 418. Embryonic Stem Cells Edited by IRINA KLIMANSKAYA AND ROBERT LANZA VOLUME 419. Adult Stem Cells Edited by IRINA KLIMANSKAYA AND ROBERT LANZA VOLUME 420. Stem Cell Tools and Other Experimental Protocols Edited by IRINA KLIMANSKAYA AND ROBERT LANZA VOLUME 421. Advanced Bacterial Genetics: Use of Transposons and Phage for Genomic Engineering Edited by KELLY T. HUGHES VOLUME 422. Two-Component Signaling Systems, Part A Edited by MELVIN I. SIMON, BRIAN R.
ROSSI VOLUME 428. Osmosensing and Osmosignaling Edited by HELMUT SIES AND DIETER HAUSSINGER VOLUME 429. Translation Initiation: Extract Systems and Molecular Genetics Edited by JON LORSCH VOLUME 430. Translation Initiation: Reconstituted Systems and Biophysical Methods Edited by JON LORSCH VOLUME 431. Translation Initiation: Cell Biology, High-Throughput and Chemical-Based Approaches Edited by JON LORSCH VOLUME 432. Lipidomics and Bioactive Lipids: Mass-Spectrometry–Based Lipid Analysis Edited by H.
Even the most experienced scientists may not be able to reliably describe features in poorly resolved regions of density maps. 5 A resolution may indicate potential problems. As such, the analysis process requires a significant investment in time and understanding the quality of the original 2D data and the reconstructed volume.
Cryo-EM, Part C: Analyses, Interpretation, and Case Studies by Grant Jensen