By Samuel P. Davis
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The velocity (depending on the molecule size) and the time-dependent changes in scattered light intensity provide information about the molecular weight of the particles. 8 nm) at 28 °C . 3. Gel Permeation Chromatography Gel permeation chromatography (GPC) is based on the differential exclusion from paths, within a suitable gel, of molecules with different molecular weights (MW). The passage is hindered more, and hence the retention time within the gel is longer, for molecules with a high degree of polymerisation and high molecular weight.
3 27 C. 2 A. 02 M. 01 R. 9 T. 5 mg, whichever is less. The ash content of each sample is estimated from the difference in the weights of the glass fibre filters before and after the heat treatment. 6. APPLICATIONS Chitosan has several characteristics that are exploited in different applications. As a polycation it is similar to metal ions in that it can bind and form complexes with negatively charged molecules such as proteins, lipids or hormones. g. the LD50 for mouse is 16 g/kg. In the body it can be slowly hydrolysed by non-specific enzymes, such as lysozyme.
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Chitosan: Manufacture, Properties, and Usage by Samuel P. Davis