Cereals for Food and Beverages: Recent Progress in Cereal by George E. Inglett PDF

By George E. Inglett

ISBN-10: 0323145620

ISBN-13: 9780323145626

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Several reagents are effective as fluorescent protein markers in situ. One of these, Acid Fuchsin, is an acid dye with marked affinity for endosperm proteins at neutral or acid pH. It has been used as a bright-field stain in many areas of cytochemistry for almost a century (see Gurr 1960 ρ 2 02), but it is much more sensitive as a fluorochrome under blue or green excitation (FC II and III). 01% aqueous solution of the dye s(adding glacial acetic acid to 1% concentration provides more intense staining).

Showing intense fluorescence in the cuticle and aleurone layers of Hinoat (Figure 28) and Betzes barley (Figure 29), and in the scutellum (Figure 30) and aleurone layer (Figure 31) of wheat. Inside Cereals—A Fluorescence Microchemical View 21 The fluorescence chemistry of the interaction between Nile Blue A and lipid deposits is not defined although Gurr (1960 ρ 299) noted a minor fluorescent component in Nile Blue A preparations, and the dye has been used to induce fluorescence in a variety of other biological systems, including muscle (Bezanilla and Horowitz 1975) and tumour cells (Bastos and Marques 1972).

Scanning electron micrographs of unmodified (Fig. 9) and crosslinked (Fig. 10) corn starch granules at 65°C.

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Cereals for Food and Beverages: Recent Progress in Cereal Chemistry and Technology by George E. Inglett


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