By David Holme, Hazel Peck
ISBN-10: 058229438X
ISBN-13: 9780582294387
Now a longtime guide of rules and methods, this article develops an figuring out of the relevance of 4 primary houses of the analyte: form, polarity, cost and dimension, to the 3 key kinds of research: separation, identity and quantification. The 3rd variation of Analytical Biochemistry has now been totally up to date in content material and structure, making it much more available to scholars studying tips on how to opt for analytical strategies and understand their scope and obstacles.
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Extra info for Analytical Biochemistry
Example text
Hence the relationship between these two units of measurement is: 1 vex A The visible region of the spectrum extends approximately over the wavelength range 400--700 nm, the shorter wavelengths being the blue end of the spectrum and the longer wavelengths the red end. Wavelengths between 400 and 200 nm make up the near ultraviolet region of the spectrum and wavelengths above 700 nm to approximately 2000 nm (2/Lm) the near infrared region. The energy associated with a particular waveform is directly related to the frequency of the radiation and therefore inversely related to the wavelength.
5 Samples for analysis The validity of a laboratory report is affected by additional factors as well as those related to the analytical method used. Of particular importance is the sample itself and the manner in which it is collected and stored prior to analysis. The collection procedure should be designed to provide a representative sample of the system under investigation and not adversely affect the analytical process. 6); in some situations ensuring that the cellular morphology is not significantly affected.
Specificity Specificity is the ability to detect only the test substance. Lack of specificity will result in false positive results if the method is qualitative and positive bias in quantitative results. The nature of any interfering substance for particular methods will be discussed in the appropriate sections but it is important to appreciate that specificity is often linked to sensitivity. It is possible to reduce the sensitivity of a method with the result that interference effects become less significant and the method is specific although less sensitive to the test substance.
Analytical Biochemistry by David Holme, Hazel Peck
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