By W. N. Aldridge (eds.)
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Additional info for A Symposium on Mechanisms of Toxicity
4 •2: ~2 26 f 0 I -4 I -3 I -2 I -1 I 0 I 2 I 3 I 4 I 5 Time (h) FIG. 6. Effect of cold-exposure (1 h at 20°C below room temperature) on the body temperature before and after the full development of a soman-hypothermia in a group of six rats. E. of mean. The cold-exposure periods are indicated by two vertical arrows connected by a horizontal bar, on top of which is given the change of body temperature due to cold-exposure. For pilo-erection and shivering see text. 38 E. Meeler The previously observed downward shift of the SPHR due to anti-ChE administration was confirmed in the present experiments.
Meister, R. A. Ronzio, W. B. Rowe, J. D. Gass, & S. L. N. Rao There is now substantial evidence that an enzyme-bound y-glutamyl phosphate intermediate is formed during glutamine synthesis. , 1956). , 1966). The well known tendency of certain y-glutamyl derivatives to cyclize to yield pyrrolidone carboxylic acid suggested experiments in which glutamine synthetase was incubated with ATP and glutamate in the absence of ammonia. , 1962). It was subsequently found that pyrrolidone carboxylic acid is indeed formed in these conditions and that the rates of cyclization are about the same with L- and D-glutamic acid.
We are particularly grateful to Dr. Anders Kjaer of the Technical University of Denmark for informing us of this research before publication, and also for providing us with generous samples of the two diastereoisomers. When these were tested as inhibitors of the enzyme, it was found that L-methionine-S-sulphoximine was about as effective as inhibiting the enzyme as was twice the concentration of L-methionine-SR-sulphoximine (Fig.
A Symposium on Mechanisms of Toxicity by W. N. Aldridge (eds.)